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KMID : 0613820030130050712
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Journal of Life Science
2003 Volume.13 No. 5 p.712 ~ p.717
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Establishment of a Leucocin A Producing Sccharomyces cerevisiae Cell
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Lee Sang-Hyun
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Abstract
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In order to establish yeast cells that produce leucocin A, a bacteriocin, the 117 bp leucocin A gene with start and stop codons was synthesized and cloned in pAUR123, a yeast vector. Transformed yeast cells showed antibacterial activity against Bacillus subtilis. The leucocin A gene was confirmed by means of PCR methods with plasmid prepared from transformed yeast cells as template and two leucocin A-specific primers. In this results, yeast cells that produce mass amounts of bacteriocin to use as food preservative or antibiotics were established.
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KEYWORD
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yeast, leucocin A, bacteriocin, Saccharomyces cerevisiae
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